Fig. 3. Simultaneous inhibition of PI3P kinase and Rac GTPase abrogates the TRIOL-induced NO^. formation in RBCs. DAF-FM associated cell fluorescence measured after a 2 h incubation of RBCs with TRIOL (2 µM) preceded by 1 h pre-treatment in the absence or in the presence of LY294002 (20 µM) or LY294002 (20 µM) plus NSC23766 (500 µM). Imagine representative of three separate experiments carried out in duplicate with comparable results.